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Peptide Mass Fingerprint (PMF)、 also known as Protein Fingerprint、 is a high-throughput protein analysis method developed in 1933 for protein identification. Endoproteases first cleave unknown target proteins into smaller peptide segments. The absolute mass of these peptides can be accurately measured using a mass spectrometer、 and a list of peptide peaks of unknown proteins can also be obtained. Compare the peak list with the theoretical peptide peak list in the protein database using bioinformatics methods. The theoretical database translates all known genomes of all organisms into proteins、 theoretically hydrolyzes proteins into small peptides、 and calculates the absolute mass of each peptide. At the same time、 the peptide mass of the unknown protein being cleaved is compared with the theoretical peptide mass of each protein encoded in the genome、 and statistical analysis of the results can find the best match
peptide quality fingerprint analysis process
After the protein in biological tissue or cell is purified、 it is separated by 2D or SDS-PAGE gel and compared by imaging technology. Select proteins with significant differences for further analysis. Degumming and enzymatic hydrolysis of selected proteins to obtain a series of peptide segments that reflect protein characteristics. Accurately measure the fingerprint of peptide mixtures through mass spectrometry to obtain the molecular weight of proteins. Searching for the selected protein in a known database provides accurate characterization
Application of peptide mass spectrometry fingerprint
With the development of analytical chemistry technology、 PMF is widely used in various fields such as drug authenticity identification、 food quality control、 and human disease diagnosis due to its high sensitivity、 good stability、 and small sample loss.
. The World Health Organization and the State Food and Drug Administration of China use PMF as a substance identification and process control technology to provide objective indicators for quality evaluationAdvantages of peptide mass spectrometry fingerprint
1. Protein identification does not rely on protein sequencing、 but rather on the quality of the peptide.
2. The process is simple and fast、 which is a classic method for protein identification using primary mass spectrometry
Limitations of peptide mass spectrometry fingerprint
1. The target protein sequence must exist in the target database 2. Suitable for single protein analysis. The presence of mixed proteins greatly increases the complexity of analysis 3. Peptides with similar quality will greatly increase the difficulty of matching
MALDI-TOF peptide quality fingerprint
Rapid and accurate identification of proteins is crucial in proteomics research.
. PMF can be measured through matrix assisted laser desorption ionization ion source (MALDI) and time of flight (TOF) mass analyzer. It is one of the most commonly used methods in proteomics researchFunction of testing report:
1. Project bidding: Issue authoritative third-party CMA/CNAS qualification report
2. Online e-commerce platform entry: Quality inspection report recognized by major e-commerce platforms 3. Used as a sales report: issuing legally effective testing reports to make consumers more confident 4. Papers and research: Provide professional personalized testing needs 5. Judicial services: providing scientific、 fair、 and accurate testing data 6. Industrial problem diagnosis: Verify the troubleshooting and correction of industrial production problemsinspection and testing process:
1. Telephone communication and confirmation of requirements
2. Recommend solutions and confirm quotations 3. Mail samples and arrange testing 4. Progress tracking and result feedback 5. Provide reports and after-sales service 6. If urgent or priority processing is requiredTesting and testing characteristics:
1. The testing industry is fully covered、 meeting different testing needs
2. Fully cover the laboratory and allocate localized testing nearby3. Engineers provide one-on-one services to make testing more accurate
4. Free initial testing、 with no testing fees charged
5. Self service order delivery for free on-site sampling
6. Short cycle、 low cost、 and attentive service 7. Possess authoritative qualifications such as CMA、 CNAS、 CAL、 etc 8. The testing report is authoritative and effective、 and is generally used in China